Anti-Human IL6 Recombinant Antibody (Sirukumab)

Name: Anti-Human IL6 Recombinant Antibody (Sirukumab)
SKU: TAB-097
Rating: 5 (1 reviews)

CAT#: TAB-097

Recombinant monoclonal antibody to IL6. Sirukumab (CNTO 136) is a human monoclonal antibody designed for the treatment of rheumatoid arthritis.

Tested Data Gene Expression

ELISA

Figure 1 Anti-Human IL6 Recombinant Antibody (TAB-097) in ELISA

ELISA analysis of TAB-097 was performed by coating with human IL6 protein (His tag). Then blocked with BSA and incubated with TAB-097. The HRP-conjugated goat anti-IgG was used as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 2 Anti-Human IL6 Recombinant Antibody (TAB-097) in WB

Western blot analysis of TAB-097 was performed by loading human IL6 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-097 and a HRP goat anti-IgG (H+L) was used as the secondary antibody (1: 3000). Chemiluminescent detection was performed.

Figure 3 Anti-Human IL6 Recombinant Antibody (TAB-097) in DB

Dot Blot analysis of TAB-097 was performed by coating with human IL6 protein (His tag).
TAB-097 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-IgG (H+L) secondary antibody (1: 3000)
NC: Negative control, PC: Positive control

❮

❯

Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72821/1350_G7_3_selected.jpg

Normal Tissue Figure 2 IHC staining of human duodenum

Immunohistochemical staining of human duodenum shows distinct cytoplasmic positivity in subsets of immune cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_A_8_2_selected.jpg

Normal Tissue Figure 3 Cerebral cortex

Neuronal cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_B_7_5.jpg

Normal Tissue Figure 4 Urinary bladder

Urothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_A_4_5.jpg

Normal Tissue Figure 5 Lymph node

Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_A_9_8.jpg

RNA Expression Figure 6 RNA cell line category: Group enriched (BJ hTERT+, GAMG)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136244-IL6

❮

❯

Specifications

Immunogen
Recombinant full length protein (Human).

Host Species
Human

Derivation
Human

Type
IgG1 - kappa

Specificity
Tested positive against native human antigen.

Species Reactivity
Human

Applications
FC, IP, ELISA, Neut, FuncS, IF, WB

CAS
1194585-53-9

Generic Name
Sirukumab

MW
144.6 kDa

Related Disease
Rheumatoid arthritis (RA) (Despite Methotrexate Therapy)

Product Property

Purity
>95.0% as determined by analysis by SDS-PAGE.

Storage
Store it under sterile conditions at -20°C upon receiving. Recommend to pack the protein into smaller quantities for optimal storage.

Applications

Application Notes
The IL6 antibody has been reported in applications of PK.

Target

Alternative Names
Sirukumab;1194585-53-9;CNTO 136;AME-19A;IL6;interleukin 6 (interferon, beta 2);IFNB2;interleukin-6;BSF2;HGF;HSF;IL 6;CDF;BSF-2;IFN-beta-2;interferon beta-2;interleukin BSF-2;hybridoma growth factor;CTL differentiation factor;B-cell stimulatory factor 2;B-

Gene ID
3569

UniProt ID
P05231

REVIEWS AND Q&AS CITATIONS RESOURCES DOWNLOADS RELATED PRODUCTS

Inquiry

Navs

Customer Review

There are currently no Customer reviews or questions for TAB-097. Click the button above to contact us or submit your feedback about this product.

Submit Your Publication

Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.

Biosimilar Overview

Please refer to Sirukumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Sirukumab.

Related Diseases

Inflammatory Bowel Diseases

Rheumatoid Arthritis

Graft-versus-Host Disease

AGE-RAGE Signaling Pathway in Diabetic Complications

Related Signaling Pathways

TNF Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-097.pdf

MSDS

MSDS - TAB-097.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

See other products for "Sirukumab"

Afuco™ Anti-IL6 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-097)
This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant monoclonal antibody to IL6. It is a human monoclonal antibody designed for the treatment of rheumatoid arthritis.

DrugMonitor™ Anti-Sirukumab Antibody (VS-1224-YC1096)
Sirukumab is a fully human IgG1 kappa monoclonal antibody selectively blocking circulating IL-6, used in trials for giant cell arteritis and rheumatoid arthritis. The DrugMonitor™ Anti-Sirukumab Antibody (VS-1224-YC1096) is an anti-drug antibody (ADA) against Sirukumab. This drug-based antibody is raised in mice immunized with the Sirukumab. The anti-Sirukumab antibody may be used in ELISA, pharmacokinetics (PK), and pharmacodynamics (PD) analyses, or serves as a reference standard in ADA assays. It also is an excellent tool for therapeutic drug monitoring, allowing to evaluate the drug efficacy and determine the drug concentration of the Sirukumab in samples.

See other products for "IL6"

Select a product category from the dropdown menu below to view related products.

CAT	Product Name	Application	Type
PSBL-613	Recombinant Human Anti-IL-6 Antibody scFv Fragment	ELISA, Neut	scFv
HPAB-0017-YC-S(P)	Human Anti-IL6 Recombinant Antibody; scFv Fragment (HPAB-0017-YC-S(P))	ELISA, Block	Human scFv
HPAB-0030LY-S(P)	Humanized Anti-IL6 Recombinant Antibody; scFv Fragment (HPAB-0030LY-S(P))	WB	scFv
HPAB-0876-FY-F(E)	Human Anti-IL6 Recombinant Antibody; Fab Fragment (HPAB-0876-FY-F(E))	ELISA	Human Fab
HPAB-1250-FY-F(E)	Mouse Anti-IL6 Recombinant Antibody; Fab Fragment (HPAB-1250-FY-F(E))	ELISA	Mouse Fab

CAT	Product Name	Application	Type
TAB-0026CL	Human Anti-IL6 Recombinant Antibody (TAB-0026CL)	Elisa, In vivo, FuncS, Block	Chimeric (mouse/human) IgG
TAB-0026CL-S(P)	Mouse Anti-IL6 Recombinant Antibody; scFv Fragment (TAB-0026CL-S(P))	Elisa, In vivo, FuncS, Block	Mouse scFv
TAB-0026CL-F(E)	Human Anti-IL6 Recombinant Antibody; Fab Fragment (TAB-0026CL-F(E))	Elisa, In vivo, FuncS, Block	Chimeric (mouse/human) Fab
TAB-670LC-F(E)	Anti-human IL6 Fab Fragment (NTS)	ELISA	Chimeric antibody (mouse/human)
VS3-WK1519	Human Anti-IL6 Recombinant Antibody (clone CLLB8)	ELISA, FC	Chimeric (mouse/human) IgG1

CAT	Product Name	Application	Type
TAB-0171CL	Mouse Anti-IL6 Recombinant Antibody (TAB-0171CL)	ELISA, Block, In vivo	Mouse IgG
TAB-0171CL-S(P)	Mouse Anti-IL6 Recombinant Antibody; scFv Fragment (TAB-0171CL-S(P))	ELISA, Block, In vivo	Mouse scFv
TAB-669LC-S(P)	Anti-human IL6 scFv Fragment (SK2)	ELISA
TAB-673LC-S(P)	Anti-human IL6 scFv Fragment	ELISA, FC
TAB-679LC-S(P)	Anti-human IL6 scFv Fragment (MH166)	ELISA

CAT	Product Name	Application	Type
TAB-0172CL	Human Anti-IL6 Recombinant Antibody (TAB-0172CL)	ELISA, Block, In vivo	Human IgG
TAB-0173CL	Human Anti-IL6 Recombinant Antibody (TAB-0173CL)	ELISA, Block, In vivo	Human IgG1
TAB-0172CL-S(P)	Human Anti-IL6 Recombinant Antibody; scFv Fragment (TAB-0172CL-S(P))	ELISA, Block, In vivo	Human scFv
TAB-0173CL-S(P)	Human Anti-IL6 Recombinant Antibody; scFv Fragment (TAB-0173CL-S(P))	ELISA, Block, In vivo	Human scFv
TAB-678LC-S(P)	Anti-human IL6 scFv Fragment (BA399)	ELISA, FC	Humanized antibody

CAT	Product Name	Application	Type
TAB-0174CL	Human Anti-IL6 Recombinant Antibody (TAB-0174CL)	ELISA, Block, In vivo	Human IgG
TAB-0175CL	Human Anti-IL6 Recombinant Antibody (TAB-0175CL)	ELISA, Block, In vivo	Human IgG
TAB-0176CL	Human Anti-IL6 Recombinant Antibody (TAB-0176CL)	ELISA, Block, In vivo	Human IgG2
TAB-671LC-S(P)	Anti-human IL6 scFv Fragment (SK2a)	ELISA	Human antibody
TAB-672LC-S(P)	Anti-human IL6 scFv Fragment (SK2b)	ELISA	Human antibody

CAT	Product Name	Application	Type
Gly-037LC	Recombinant Anti-Human IL6 Antibody (Fab glycosylation)	ELISA	Humanized antibody

CAT	Product Name	Application	Type
Gly-197LC	Recombinant Anti-Human IL6 Antibody (Non-glycosylated)	ELISA	Humanized antibody

CAT	Product Name	Application	Type
MOB-0632CT	Recombinant Mouse anti-Human IL6 Monoclonal antibody (6708)	ELISA, WB
HPAB-588-FY	Human Anti-IL6 Recombinant Antibody (HPAB-588-FY)	FC, ELISA	Human IgG2
HPAB-591-FY	Human Anti-IL6 Recombinant Antibody (HPAB-591-FY)	Inhib	Human IgG1
FN-242CQ	Rat Anti-Il6 Recombinant Antibody (clone MP5-32C11)	FuncS	Rat IgG2a, κ
HPAB-1758-FY	Rat Anti-IL6 Recombinant Antibody (HPAB-1758-FY)	ELISA	Rat IgG

CAT	Product Name	Application	Type
BRD-0284MZ	Chicken Anti-Interleukin-6 Polyclonal IgY	WB, Indirect ELISA	Chicken antibody

CAT	Product Name	Application	Type
NEUT-1450CQ	Mouse Anti-IL6 Recombinant Antibody (clone CBL596)	Neut	Mouse IgG2a
NEUT-1451CQ	Mouse Anti-IL6 Recombinant Antibody (clone CBL661)	Neut	Mouse IgG2b
NEUT-1452CQ	Mouse Anti-IL6 Recombinant Antibody (clone CBL553)	WB, Neut	Mouse IgG2a
NEUT-1453CQ	Mouse Anti-IL6 Recombinant Antibody (clone CBL615)	WB, Neut	Mouse IgG2b
NEUT-1454CQ	Mouse Anti-IL6 Recombinant Antibody (clone 1X45)	IHC, Neut	Mouse IgG2

CAT	Product Name	Application	Type
NEUT-1474CQ	Rat Anti-Il6 Recombinant Antibody (clone 32C11)	Block, ELISA, WB	Rat IgG2a

CAT	Product Name	Application	Type
MOR-1814	Rabbit Anti-IL6 Recombinant Antibody (clone DS1814AB)	WB	Rabbit IgG
MOR-4277	Rabbit Anti-IL6 Recombinant Antibody (clone SI360DS)	ELISA	Rabbit IgG
MOR-4622	Rabbit Anti-IL6 Recombinant Antibody (clone TH135DS)	WB, ELISA	Rabbit IgG
MOR-4623	Rabbit Anti-IL6 Recombinant Antibody (clone TH136DS)	WB	Rabbit IgG

CAT	Product Name	Application	Type
MHC-LC3429	PE-B8/Human IL6 (ILKQKIALD) MHC Tetramer	FCM
MHC-LC3430	APC-B8/Human IL6 (ILKQKIALD) MHC Tetramer	FCM

CAT	Product Name	Application	Type
NS-043CN	Human Anti-IL6 Recombinant Antibody (NS-043CN)	ELISA, WB	Human IgG1
NS-043CN-F(E)	Human Anti-IL6 Recombinant Antibody; Fab Fragment (NS-043CN-F(E))	ELISA, WB	Human Fab
NS-043CN-S(P)	Human Anti-IL6 Recombinant Antibody; scFv Fragment (NS-043CN-S(P))	ELISA, WB	Human scFv

CAT	Product Name	Application	Type
AFC-TAB-239	Afuco™ Anti-IL6 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-239)	FuncS, IF, Neut, ELISA, FC	ADCC enhanced antibody
AFC-TAB-097	Afuco™ Anti-IL6 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-097)	FC, IP, ELISA, Neut, FuncS, IF	ADCC enhanced antibody
AFC-TAB-H26	Afuco™ Anti-IL6 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H26)	Neut, ELISA, IF, IP, FuncS, FC	ADCC enhanced antibody
AFC-TAB-253	Afuco™ Anti-IL6 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-253)	ELISA, FC, IP, FuncS, IF	ADCC enhanced antibody

CAT	Product Name	Application	Type
HPAB-1562-FY-S(P)	Human Anti-IL6 Recombinant Antibody; scFv Fragment (HPAB-1562-FY-S(P))	WB, ELISA, Neut	Human scFv
HPAB-1563-FY-S(P)	Human Anti-IL6 Recombinant Antibody; scFv Fragment (HPAB-1563-FY-S(P))	WB, ELISA, Neut	Human scFv
HPAB-1564-FY-S(P)	Human Anti-IL6 Recombinant Antibody; scFv Fragment (HPAB-1564-FY-S(P))	WB, ELISA, Neut	Human scFv
HPAB-1105WJ-F(E)	Mouse Anti-IL6 Recombinant Antibody (clone CLB8); Fab Fragment	Neut, ELISA	Mouse Fab
HPAB-S0076-YC-F(E)	Rabbit Anti-IL6 Recombinant Antibody; Fab Fragment (HPAB-S0076-YC-F(E))	ELISA, WB, Block, FuncS	Rabbit Fab

CAT	Product Name	Application	Type
HPAB-S0166-YC	Camelid Anti-IL6 Recombinant Single Domain Antibody (HPAB-S0166-YC)	ELISA	Camelid VHH
HPAB-S0167-YC	Camelid Anti-IL6 Recombinant Single Domain Antibody (HPAB-S0167-YC)	ELISA	Camelid VHH
HPAB-S0168-YC	Camelid Anti-IL6 Recombinant Single Domain Antibody (HPAB-S0168-YC)	ELISA, Inhib	Camelid VHH
HPAB-S0169-YC	Camelid Anti-IL6 Recombinant Single Domain Antibody (HPAB-S0169-YC)	ELISA, Inhib	Camelid VHH
HPAB-S0170-YC	Camelid Anti-IL6 Recombinant Single Domain Antibody (HPAB-S0170-YC)	ELISA, Inhib	Camelid VHH

CAT	Product Name	Application	Type
VS-0424-XY151	AbPlus™ Anti-IL6 Magnetic Beads (AS16)	IP, Protein Purification

CAT	Product Name	Application	Type
VS-1024-XY279	Mouse Anti-NHP IL6 Recombinant Antibody (clone 6708)	WB, ELISA	Mouse IgG1

CAT	Product Name	Application	Type
VS-0225-XY146	CytoStream™ Mouse Anti-IL6 Recombinant Antibody (VS-0225-XY146)	FC	Mouse IgG1

CAT	Product Name	Application	Type
VS-0425-FY11	Human Anti-IL6 (clone BA001) scFv-Fc Chimera	ELISA, Block	Human IgG1, scFv-Fc

CAT	Product Name	Application	Type
VS-0425-YC526	Recombinant Anti-IL6 Vesicular Antibody, EV Displayed (VS-0425-YC526)	ELISA, FC, Neut, Cell-uptake

CAT	Product Name	Application	Type
VS-0525-XY3537	Anti-Mouse IL6 Immunohistochemistry Kit	IHC
VS-0525-XY3538	Anti-Human IL6 Immunohistochemistry Kit	IHC

Specific Inquiry

Size:

Conjugation:

Endotoxin:

Purity:

Formats:

Isotype Switching:

Fc Engineering:

Modalities:

Add to compare

Request
Custom Production

See Our Custom Production in Action

Figure 1 Anti-HBV Recombinant Antibody (TAB-147) in SDS-PAGE

Download the full case study

Popular Products

Figure 2 Anti-S. Aureus Recombinant Antibody (TAB-029) in HPLC

Anti-S. Aureus Recombinant Antibody (TAB-029) (CAT# TAB-029)
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC

Figure 5 Anti-RSV glycoprotein F Recombinant Antibody (TAB-709) in ELISA

Anti-RSV glycoprotein F Recombinant Antibody (TAB-709) (CAT# TAB-709)
Application: IF, IP, Neut, FuncS, ELISA, FC, WB

Figure 2 Anti-alpha 4 beta 7 Antibody (TAB-H75) in Dot Blot

Anti-Human ITGA4+ITGB7 Recombinant Antibody (TAB-H75) (CAT# TAB-H75)
Application: FC, IP, ELISA, Neut, FuncS, IF, WB

Figure 2 Anti-Human GAST Recombinant Antibody (TAB-300CL) in HPLC

Anti-Human GAST Recombinant Antibody (XPA067.06) (CAT# TAB-300CL)
Application:

Figure 3 Anti-Human HSP90 Recombinant Antibody (TAB-368CL) in ELISA

Anti-Human HSP90 Recombinant Antibody (CAT# TAB-368CL)
Application:

Figure 5 Recombinant Human Anti-IAV HA Antibody (5J8) (PABL-207) in Dot Blot

Recombinant Human Anti-IAV HA Antibody (5J8) (CAT# PABL-207)
Application: FuncS

Figure 2 Recombinant Mouse Anti-uPAR Antibody (PABL-355) in ELISA

Mouse Anti-PLAUR Recombinant Antibody (PABL-355) (CAT# PABL-355)
Application: WB, ELISA, FuncS

Figure 1 Anti-Human CD200R Protein Antibody in ELISA

Human Anti-CD200R1 Recombinant Antibody (clone OX108) (CAT# PABC-020)
Application: WB, FuncS

Figure 3 Mouse Anti-VEGF Recombinant Antibody (PABW-130) in ELISA

Mouse Anti-VEGF Recombinant Antibody (clone A4.6.1) (CAT# PABW-130)
Application: Neut, ELISA, Inhib, ICC, WB

Figure 3 Human Anti-MS4A1 Recombinant Antibody (PABL-422) in Flow Cytometry

Human Anti-MS4A1 Recombinant Antibody (clone 2F2) (CAT# PABL-422)
Application: WB, ELISA, FuncS

Figure 1 Mouse Anti-α4β7 Recombinant Antibody (PABL-746) in ELISA

Mouse Anti-α4β7 Recombinant Antibody (PABL-746) (CAT# PABL-746)
Application: FC

Figure 3 Anti-FOLR1 Antibody (TAB-020CQ) in Dot Blot

Mouse Anti-FOLR1 Recombinant Antibody (TAB-020CQ) (CAT# TAB-020CQ)
Application: ELISA, FC, WB, IHC

Figure 5 Mouse Anti-HAVCR1 Recombinant Antibody (TAB-664CT) in DB

Mouse Anti-HAVCR1 Recombinant Antibody (TAB-664CT) (CAT# TAB-664CT)
Application: FC, IB, Block, Inhib, FuncS, ELISA, FACS, IP, IF

Figure 1 Anti-Human SLC1A5 Antibody (TAB-1010CLV) in FC

Anti-Human SLC1A5 Recombinant Antibody (TAB-1010CLV) (CAT# TAB-1010CLV)
Application: FC, ADCC, CDC

Figure 4 Anti-Human KLRC1 Recombinant Antibody (TAB-437CQ, Fc Silenced) in ELISA

Anti-Human KLRC1 Recombinant Antibody (TAB-437CQ) (CAT# TAB-437CQ)
Application: ELISA, IHC, FC, IP, IF, FuncS

Figure 2 Mouse Anti-C5 Recombinant Antibody (HPAB-0128CQ) in ELISA

Mouse Anti-C5 Recombinant Antibody (clone BB5.1) (CAT# HPAB-0128CQ)
Application: FC, IA, IF, IP, IHC, FuncS

Figure 2 Mouse Anti-CRP Recombinant Antibody; Fab Fragment (HPAB-M0039-YC-F(E)) in ELISA

Mouse Anti-CRP Recombinant Antibody (clone P24); Fab Fragment (CAT# HPAB-M0039-YC-F(E))
Application: ELISA

Figure 2 Recombinant Human Anti-HA Antibody (HPAB-N0125-YC) in ELISA

Recombinant Human Anti-HA Antibody (S9-1-10/5-1) (CAT# HPAB-N0125-YC)
Application: ELISA, FuncS, Neut, IF, WB, EM, Inhib, IHC

Figure 2 Human Anti-EBV gB Recombinant Antibody (Clone AMMO4) (PABS-0039) in WB

Human Anti-EBV gB Recombinant Antibody (Clone AMMO4) (CAT# PABS-0039)
Application: ELISA, FuncS

Figure 4 Human Anti-FZD5 Recombinant Antibody (PABJ-0145) in ELISA

Human Anti-FZD5 Recombinant Antibody (PABJ-0145) (CAT# PABJ-0145)
Application: ELISA, Inhib, FuncS

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.