Anti-Human IL6 Recombinant Antibody (Sirukumab) (CAT#: TAB-097)

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• Tested Data
• Gene Expression
• Datasheet
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ELISA

Figure 1 Anti-Human IL6 Recombinant Antibody (TAB-097) in ELISA

ELISA analysis of TAB-097 was performed by coating with human IL6 protein (His tag). Then blocked with BSA and incubated with TAB-097. The HRP-conjugated goat anti-IgG was used as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

WB

Figure 2 Anti-Human IL6 Recombinant Antibody (TAB-097) in WB

Western blot analysis of TAB-097 was performed by loading human IL6 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-097 and a HRP goat anti-IgG (H+L) was used as the secondary antibody (1: 3000). Chemiluminescent detection was performed.

DB

Figure 3 Anti-Human IL6 Recombinant Antibody (TAB-097) in DB

Dot Blot analysis of TAB-097 was performed by coating with human IL6 protein (His tag).
TAB-097 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-IgG (H+L) secondary antibody (1: 3000)
NC: Negative control, PC: Positive control

Subcellular Location

Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72821/1350_G7_3_selected.jpg

Normal Tissue

Figure 2 IHC staining of human duodenum
(Immunohistochemical staining of human duodenum shows distinct cytoplasmic positivity in subsets of immune cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_A_8_2_selected.jpg

Figure 3 Cerebral cortex
(Neuronal cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_B_7_5.jpg

Figure 4 Urinary bladder
(Urothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_A_4_5.jpg

Figure 5 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_A_9_8.jpg

RNA Expression

Figure 6 RNA cell line category: Group enriched (BJ hTERT+, GAMG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136244-IL6

 Datasheet

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Product Notes

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.